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GeoChembio/MetaPathogen/plant pathogens/doublestranded RNA viruses Back to all files MetaPathogen pathogens Data and reports Pathogen survival Famous people deaths Human pathogens Bacterial pathogens Fungal pathogens Protozoan pathogens Helminths DNA Viruses ssRNA() Viruses ssRNA() Viruses Reo and Retroviruses Plant pathogens BacterialThis virus family is the largest family of doublestranded RNA viruses, and perhaps the most understood of their kind They have been identified in a wide variety of organisms, found in everything from an arachnid, a plant, fungi, reptiles, mammals, and more In humans, this viral family is responsible for the commonly known Rotavirus The Rotavirus is passed from fecalA novel virus was detected in ornamental plants of Primula malacoides Franch exhibiting typical yellowedge symptoms Two doublestranded RNA (dsRNA) segments, of 2390 bp and 2344 bp, respectively, were extracted from plant tissues, and these same dsRNAs were detected from purified virions of about 35 nm in diameter The two dsRNAs, putatively encoding partitivirusrelated RNAdependent RNA

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Double stranded rna is found in which plant virus

Double stranded rna is found in which plant virus-Plant RNA viruses are obligate intracellular parasites with singlestranded (ss) or doublestranded RNA genome(s) generally encapsidated but rarely enveloped For viruses with ssRNA genomes, the polarity of the infectious RNA (positive or negative) and the presence of one or more genomic RNA segments are the features that mostly determine the molecular mechanismsMost plant viruses are singlestranded RNA or doublestranded RNA viruses Common plant viruses include mosaic viruses, spotted wilt viruses, and leaf curl viruses Plant viruses are typically spread by either horizontal or verticle transmission Viroids are singlestranded molecules of RNA that cause plant diseases that lead to underdevelopment Satellite viruses

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Replication occurs in host cytoplasm and converts ssmRNA to dsgenomic RNA But dsRNA is a kind of molecule that cells do not produce, and eukaryotic hosts have various antiviral systems that detect and inactivate dsRNA To circumvent this defenses, many dsRNA viruses are replicating their RNA inside icosahedral capsids The RNA polymerasesAnalysis of doublestranded RNA for plant virus diagnosis Author(s) Valverde, R A;In this study, we report the first identification of an RSS from a plant doublestranded RNA (dsRNA) virus Pns10, encoded by S10 of Rice dwarf phytoreovirus (RDV), exhibited RSS activity in coinfiltration assays with the reporter green fluorescent protein (GFP) in transgenic Nicotiana benthamiana line 16c carrying GFP The other gene segments of the RDV genome did not have

During the past decade 16 viruses which possess doublestranded ribonucleic acid (dsRNA) genomes have been found in vertebrate, invertebrate, bacterial, higher plant and fungal hosts Although once considered a small unique class of viruses, it is now apparent that dsRNA viruses may be of common occurrence especially among the mycoviruses (viruses of fungi) andDNA viruses are relatively rare in plants, compared to their RNA counterparts Like most viruses, the genomes of most single stranded DNA viruses are small, encoding only a few proteins, and are therefore dependent on host cell factors for replication Double stranded DNA viruses only infect lower species of plants, such as algae These viruses are huge dsDNA virusesBy the detection of doublestranded RNA (dsRNA) viral replication intermediates and their cleavage by an RNase III en zyme called Dicer that produces short ( 22nt) RNA molecules, known as short interfering RNAs (siRNAs) (for review, see ref 3) Recent discoveries in both plants and animals have established that similar small RNA molecules, known as microRNAs, are produced

Voinnet et al, 1999;Key words BCV/dsRNA/plant virus detection 47 Molecular Cloning of the Doublestranded RNA of Beet Cryptic Viruses By J F ANTONIW, 1,2 H J M LINTHORST, 1 R F WHITE 3 AND J F BOL' ~ *Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands and Departments of 2Biochemistry and 3plant Pathology, RothamstedIt has been found within viral capsid in the reoviruses of animals and in the wound tumour virus and rice dwarf viruses of plants Singlestranded RNA;

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Doublestranded RNA fijivirus associated with symptom development MENGAO JIA1, YONGQIANG LI1, LEI LEI1, DIANPING DI2, HONGQIN MIAO2 AND ZAIFENG FAN1,* 1State Key Laboratory of Agrobiotechnology and Department of Plant Pathology, China Agricultural University, Beijing , China 2Plant Protection Institute, Hebei Academy of Agricultural and ForestryDoublestranded RNA (dsRNA) is RNA with two complementary strands, similar to the DNA found in all cells, but with the replacement of thymine by uracil dsRNA forms the genetic material of some viruses (doublestranded RNA viruses) Doublestranded RNA, such as viral RNA or siRNA, can trigger RNA interference in eukaryotes, as well asRosa et al, 18) These dsRNA molecules are recognized and cleaved by Dicer like proteins present in the cell to generate siRNAs After

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Doublestranded RNA viruses Doublestranded (ds) RNA viruses are a diverse group of viruses that vary widely in host range (animals, plants, fungi, and bacteria), genome segment number (one to twelve) and virion organization (Tnumber, capsid layers or turrets) Wikipedia Pandoraviridae Family of doublestranded DNA viruses that infect amoebaeThe doublestranded (ds)RNA viruses represent a diverse group of viruses that vary widely in host range (humans, animals, plants, fungi, and bacteria), genome segment number (one to twelve), and virion organization (Tnumber, capsid layers, or turrets) Members of this fascinating group include the rotaviruses, renowned globally as the commonest cause of gastroenteritis inDoubleStranded RNA Viruses dsRNA viruses are a widely distributed group of viruses that infect practically all organisms, from the mammalian reoviruses to the bacteriophage ϕ6, and include plant and fungal viruses From Advances in Virus Research, 13 Download as PDF About this page

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The percentage of the total mapped reads and the percentage of the reference coverage can be found in Isolation and analysis of doublestranded RNA from virusinfected plant and fungal tissue Phytopathology 69 (8) 854 Accessed 3 January 19 View Article Google Scholar 23 Roossinck MJ, Saha P, Wiley GB, Quan J, White JD et al (10) Ecogenomics usingDerived from RNA virus replication, dsRNAs synthesized by plant endogenous RNAdependent RNA polymerase, dsRNAs of singlestranded RNAs that form hairpinloop secondary structures (Baulcombe 04;Singlestranded RNA Most plant viruses are classified as singlestranded RNA or doublestranded RNA virus particles Cause various types of plant diseases These diseases do not typically result in plant death 5 Plant diseases produce symptoms such as Ring spots Mosaic pattern development Leaf yellowing and distortion Deformed growth Some plant viruses are not limited to one particular plant

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Here are many viruses with a doublestranded DNA genome that are known to infect mammals They are subdivided into seven virus families Hepadnaviridae, Polyomaviridae, Papillomaviridae, Adenoviridae, Herpesviridae, Poxviridae and Asfarviridae WiRNA silencing functions as a potent antiviral pathway in plant and animal systems 1,2,3,4,5,6 and is known to be triggered by the accumulation of doublestranded viral RNA (dsRNA) The viralDoubleStranded RNA Virus dsRNA viruses are a widely distributed group of viruses that infect practically all organisms, from the mammalian reoviruses to the bacteriophage ϕ6, and include plant and fungal viruses From Advances in Virus Research, 13 Download as PDF

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Plant Viral DoubleStranded RNA Annual Review of Phytopathology Vol (Volume publication date September 1984) PlantParasitic Nematodes and Food Security in SubSaharan Africa Danny L Coyne, Laura Cortada, Johnathan J Dalzell, Abiodun O ClaudiusCole, Solveig Haukeland, Nessie Luambano, Herbert Talwana Vol 56, 18 Abstract Figures PreviewHexagonal viruslike particles (VLPs) measuring 45 nm across were detected in mycelial extracts from Trichothecium roseum Himachal strain, the source fungus for the production of Tpoly (Trichothecium polysaccharide), a known inhibitor of plant viruses VLPs were found to contain doublestranded RNA (ds RNA) and the purified ds RNA was capable of inhibiting tobacco mosaic virusMonoclonal antibodies to doublestranded RNA (dsRNA) are described for use as a universal diagnostic tool to detect infection in plants by RNA viruses Crude nucleic acid extracts from plants infected with one of 25 different viruses were examined by sandwichELISA and immunoblotting In comparison to the corresponding controls elevated dsRNA concentrations were found

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DoubleStranded DNA Viruses Doublestranded DNA viruses of plants are rare, and only infect lower plants, such as algae These viruses (family Phycodnaviridae) are huge dsDNA viruses with genomes ranging from 160 to 560 kb with up to 600 proteinencoding genes, making them distinctly different from viruses infecting higher plants They are found in aqueousINTRODUCTION Virus infection &Nonretroviral RNA virus sequences (NRVSs) have been found in the chromosomes of vertebrates and fungi, but not plants Here we report similarly endogenized NRVSs derived from plus, negative, and doublestranded RNA viruses in plant chromosomes These sequences were found by searching public genomic sequence databases, and, importantly, most NRVSs were

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Replication – A synonym Virus is a nucleoprotein having RNA or DNA as a genetic material RNA or DNA may be ss or ds, ssRNA may be ve or –ve sense Most of plant virus infect a limited number of different plant species and a few have a wide host range Viruses do not produce any kind of reproductive structure, theyKeywords doublestranded RNA, patterntriggered immunity, receptor recognition, subcellular localization, vesicle transport, virus infection INTRODUCTION Recognition of viral doublestranded (ds)RNA is a major mechanism in plant antiviral defence via RNA silencing and patterntriggered immunity (PTI) dsRNA is a common pattern formed during replication of RNA and DNA virusesRNA interference (RNAi) is a genetic regulatory system that functions to silence the activity of specific genes RNAi occurs naturally, through the production of nuclearencoded premicroRNA (premiRNA), and can be induced experimentally, using short segments of synthetic doublestranded RNA (dsRNA)

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Topical application of doublestranded RNA molecules efficient plant virus control In this work, doublestranded RNA (dsRNA) molecules deriving from sequences of the C4 and V2 genes of TYLCVMild were produced in vitro and topically applied onto tomato plants along with the virus (via agroinfiltration) DsC4 and dsV2 application reduced disease incidence to 23 and 46 %,It is found in most of the RNA viruses eg tobacco mosaic virus, influenza virus, poliomyelitis, bacteriophage MS2, Avian leukemia virus On the Basis of Presence of Envelope The envelopeDoublestranded (ds) RNA viruses are a diverse group of viruses that vary widely in host range (humans, animals, plants, fungi, and bacteria), genome segment number (one to twelve) and virion organization (Tnumber, capsid layers or turrets) Members of this group include the rotaviruses, known globally as a common cause of gastroenteritis in young children, and bluetongue virus, an

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Host Factors Against Plant Viruses Garcia Ruiz 19 Molecular Plant Pathology Wiley Online Library

Plant viruses of the recently recognized family Amalgaviridae have monopartite doublestranded (ds) RNA genomes and encode two proteins an RNAdependent RNA polymerase (RdRp) and a putative capsid protein (CP) Whereas the RdRp of amalgaviruses has been found to be most closely related to the RdRps of dsRNA viruses of the family Partitiviridae,A double stranded or in a genome isolated from a virus was found to contain 15% yourself What percentage of guanine should exist in this viruses?Interaction of Ligustrum Mosaic Virus With the Plant RNA Interference Machinery Mapping of sRNA reads from the virusinfected privet leaf sample HYT24 on the reconstructed LigMV genome revealed that viral sRNAs constitute 22% of total (plant viral) –25 nt reads Only negligible amounts of viral sRNA reads were found in the nonsymptomatic privet leaf

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Preparations of doublestranded (ds) RNAs extracted from naturally infected Vicia faba Linn growing in Hangzhou, Zhejiang Province, Eastern China displayed 3 dominant bands (FaR1, FaR2, and FaR3) FaR2 and FaR3 were found to be identical to the genomic dsRNAs of a recently reported Vicia cryptic virus (VCV) The positive strand of FaR1 contained two large open reading framesThe members of the families Reoviridae and Birnaviridae possess a doublestranded, segmented RNA genome Similar molecular properties are found in plant viruses, such as partitiviruses The genome of birnaviruses, which are not pathogenic for humans, has two genome segments In contrast, the genomes of reoviruses encompass 9–12 RNA segmentsAs the majority of plant viruses have an RNA genome, a common approach is to extract the doublestranded RNA (dsRNA) replicative form, to enrich the replicating virus genetic material over the host background The traditional dsRNA extraction is timeconsuming and labourintensive We present an alternative method to enrich dsRNA from plant extracts using anti

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Plant RNA Viruses and RNA Warfare Plant viruses are most often made from RNA The plant takes pieces of viral RNA and, from it, manufactures double stranded RNA pieces with a hairpin turn connecting the two strands (see picture) The double strands of RNA are made by copying the RNA with the enzyme polymerase Double stranded RNA can travel in the cell without attachingFound) coding region methylated) not dependent on ongoing translation) fully reversible during meioses PTGS can also be induced by homologous DNA, with no promoter sequence (minimal length 300 bp) „ectopic pairing" aberrant RNA Grant,S, 1999, Cell, Vol 96, 303–306 N clevelandii TMV systemic infection with strong symptoms Plants use PTGS as a defence against virusesJordan, R L Author Affiliation see more details of plant virus diseases is discussed The advantages and disadvantages of this technique are presented, and a simplified version of the technique is described ISSN Record Number Language of text

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High Plains disease of maize and wheat were found to be associated with viruses that share several characteristics They all have singlestranded multipartite RNA genomes of negative orientation In some cases, double membranebound viruslike particles of 80 to 0 nm in diameter were found in infected tissue Furthermore, at least five of these viruses were shown最も人気のある！ double stranded rna is found in which virus RNAdependent RNA polymerase 6 (RDR6) is an essential component of RNAi, which plays an important role in the resistance against viruses in the model plants We found previously that rice RDR6 (OsRDR6) functioned in the defense against Rice stripe virus (RSV), and Rice Dwarf Phytoreovirus (RDV)Most mycoviruses have a doublestranded RNA (dsRNA) genome, therefore dsRNAdetection is frequently used as a first step to identify virus infection In relation with MVX 23 dsRNAs species have been described, occurring in variable number and combination in diseased mushrooms The aim of our experiments was to find out whether dsRNAimmunoblotting can be used to detect

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